Flow cytometry

DESCRIPTION:

Cell analysis and cell sorting by flow cytometry is a well established technique whereby rapid measurements of multiple cellular parameters such as light scatter and fluorescence are made on particles or cells as they flow through a sensing point forming the basis for selected populations to be analyzed and/ or sorted out. The CMM flow cytometry facility is equipped with:

Cell sorters:

BD Influx with 3 lasers (488, 561 and 641 nm)

Up to six different cell populations can be sorted out simultaneously into tubes, or one population onto plates (including index sorting).

Sony SH800 with 4 lasers (488, 561, 638, and 405 nm)

Up to two different populations can be sorted out simultaneously into tubes, or one population onto plates (including index sorting).

Cytek Aurora CS with 5 lasers (488, 561, 641 and 355 nm)

Up to 6 different cell populations can be sorted out simultaneously into tubes, or one population onto plates (including index sorting).

Analyzers:

BD FACSVerse;  equipped with 3 lasers: (488nm, 635nm, and 405nm).

BD LSR Fortessa;  equipped with 5 lasers: (488, 552, 640, 405, and 350 nm).

Cytek Aurora; equipped with 5 lasers: (488nm, 635nm, 560nm, 405nm and 355 nm)

Cytek Aurora; equipped with 4 lasers: (488nm, 635nm, 560nm, and 405nm).

Cytek Aurora; equipped with 3 lasers: (488nm, 635nm and 405nm).

Key applications include multicolor immmunophenotyping, cell cycle analysis, intracellular cytokine detection, apoptosis, cell proliferation analysis, and intracellular calcium measurement. Assistance and guidance in planning your experiments as well as suggestions for helping you achieve optimal results are available.

CONTACT:

Annika van Vollenhoven

Location: CMM floor 03, room 064

E-mail: annika.van.vollenhoven@ki.se

Phone: +46 8-517 75693

Address:

Flow Cytometry Facility

CMM L8:03

171 76 Stockholm